Covalent-Bond-Based Immobilization Approaches for Single-Molecule Fluorescence

Single molecule fluorescence approaches shed light on intracellular RNAs.

SnapShot: Single-Molecule Fluorescence

Common uorescent agents include organic uorophores (e.g., Cy5), uorescent proteins (e.g., GFP), and semiconductor quantum dots each with different size, photobleaching, blinking, and labeling ef ciency properties. Wideeld detection with EMCCD camera enables parallel measurements. The temporal resolution is >ms, and the working concentration is limited to nM or less. Confocal detection from an e...

Direct imaging of covalent bond structure in single-molecule chemical reactions.

Observing the intricate chemical transformation of an individual molecule as it undergoes a complex reaction is a long-standing challenge in molecular imaging. Advances in scanning probe microscopy now provide the tools to visualize not only the frontier orbitals of chemical reaction partners and products, but their internal covalent bond configurations as well. We used noncontact atomic force ...

Single molecule fluorescence control for nanotechnology.

Nanoscience and nanotechnology are providing fresh approaches to engineer materials with practical applications such as molecular-sized therapeutic and diagnostic devices, drug delivery vehicles, environmental sensors, high-rate manufacturing platforms, and self-assembling nanomachines. In many cases, researchers are mimicking biological systems that easily achieve molecular scale control throu...

Site-Directed Covalent Immobilization